Journal:

Article Title: RNA Interference in the Moss Physcomitrella patens 1

doi: 10.1104/pp.103.024901

Figure Lengend Snippet: Transient RNAi in P. patens. NLS-4 (a transgenic moss line expressing nuclearly localized GFP) was cobombarded with dsRed2 and either GUS-RNAi or LUC-RNAi constructs. Transformed cells are marked with dsRed2 fluorescent protein. The nuclear GFP signal is reduced when the cell is transformed with GUS-RNAi (top row) and unaffected in neighboring untransformed cells (arrows). Transformation with LUC-RNAi has no effect on the nuclear GFP signal (bottom row). Images were collected with the Zeiss confocal microscope.

Article Snippet: We used the software provided with the Zeiss microscope system to perform linear unmixing of data in which both chromophores were expressed.

Techniques: Transgenic Assay, Expressing, Construct, Transformation Assay, Microscopy

Journal:

Article Title: RNA Interference in the Moss Physcomitrella patens 1

doi: 10.1104/pp.103.024901

Figure Lengend Snippet: Time course of transient RNAi. The intensity of nuclear GFP signal in cells transformed with each RNAi construct is shown for the indicated time points after particle bombardment. At least 15 cells were measured for each construct. Error bars indicate se. Statistical analysis was carried out with the Student's t test for unpaired data with unequal variance. The asterisk shows data sets are statistically different from LUC-RNAi with a t-probability of <0.001. #, A t-probability of 0.0056. Images were collected with the Leica confocal microscope.

Article Snippet: We used the software provided with the Zeiss microscope system to perform linear unmixing of data in which both chromophores were expressed.

Techniques: Transformation Assay, Construct, Microscopy